Mullerian Inhibiting Substance (AMH)

Code

4089

Principle

Anti-Müllerian hormone (AMH) is a glycoprotein, which circulates as a dimer composed of two identical 72 kDa monomers that are linked by disulfide bridges. AMH belongs to the transforming growth factor-β family. AMH concentrations in adult women reflect the number of small follicles entering the growth phase of their life cycle, which is proportional to the number of primordial follicles that still remain in the ovary, or the ovarian reserve.AMH decreases throughout a woman’s reproductive life, which reflects the continuous decline of the oocyte/follicle pool with age and, accordingly, ovarian aging.

AMH has been used in the evaluation of ovarian reserve primarily to predict an infertile woman’s response to controlled ovarian stimulation.Research studies have also demonstrated that AMH may be used to estimate the time to menopause for an individual woman, as AMH has been found to be a good indicator of reproductive aging.Additionally, studies have shown that AMH can be used to diagnose and monitor women with polycystic ovary syndrome (PCOS), and that AMH concentrations are elevated in normogonadotropic anovulatory women with PCOS.

Preanalytical

1 mL Serum Refrigerated

Turn Around Time

3 days

Methodology

Chemiluminescent immunoassay (CLIA)

Limitations

Patients who have been regularly exposed to animals or have received immunotherapy or diagnostic procedures utilizing immunoglobulins or immunoglobulin fragments may produce antibodies, e.g. HAMA, that interfere with immunoassays.

Other potential interferences in the patient sample could be present and may cause erroneous results in immunoassays. Some examples that have been documented in literature include rheumatoid factor, endogenous alkaline phosphatase, fibrin, and proteins capable of binding to alkaline phosphatase.
Carefully evaluate the results of patients suspected of having these types of interferences

All results should be interpreted in light of the total clinical presentation of the patient, including: symptoms, clinical history, data from additional tests and other appropriate information.